Using Sequencing Technique for Diagnostic Different Species of Genus Rhizobium Which Isolated from Legume Plants

Samples of the root nodules were collected to isolate different species genus Rhizobium from several leguminous plants; Trigonella foenum-graecum, Medicago sativa, Lens culinaris, Vigna mungo, Vicia faba, Phaseolus vulgaris, and Cicer arietinum, based on their morphological, cultural, biochemical characteristics, in addition identification each at level by amplified polymerase chain reaction (PCR) using sequencing nitrogenous bases 16S rRNA gene, it was identified as Sinrhizobium meliloti, Bradyrhizobium elkanii, leguminosarium biovar viciae, phaseoli Mesorhizobium cicero, respectively, (with exception bacteria isolated culinaris) showed an agreement with GenBank isolates percentages ranging between (96-98) percent, well drawing phylogenetic tree determine extent evolution these convergence original international standard isolates.